autograft of neonatal mouse ovary afetr vitrification

P-37: Vitrification of Mouse Ovary in Presence of Zinc in Vitrification Medium: Histological Evaluation

Background Whole ovary cryopreservation has been proved to be a feasible technique in fertility preservation. The normal ovarian cortex contains a large population of primordial follicles, this characteristic make them far more tolerant to cryoinjury. Researchers have used different cryoprotectants and various techniques to improve cryopreservation. It is plausible that inclusion of an antioxid...

Steroid Production and Follicular Development of Neonatal Mouse Ovary during In Vitro Culture

O-9: The Peroxidation Indices during Vitrification of Bovine Ovary

Background: Cryopreservation of ovary for preserving germ cell line is going to be an important issue. However, the physical and chemical stresses during cryopreservation are the most important factors that may influence the post freezing tissue viability and quality. Vitrification is a new approach to bypass the impacts of both stresses for gametes and embryos and it is also suggested for ovar...

Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device

BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...

Which stage of mouse embryos is more appropriate for vitrification?

Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

P-46: Vitrification of Mouse Ovaries Under Magnefic Field

Background The aim of this study was to investigate the effect of applying the 1-mT magnetic field (MF) in the ovarian tissue vitrification. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice randomly were divided into 5 groups: V1: Ovaries that immediately after leaving of the mice body, were examined histologically (control group), V2: Ovaries that withou...